pLVX-MetLuc慢病毒载体使用说明

编号 北京华越洋VECT231189 载体名称 pLVX-MetLuc

pLVX-MetLuc载体基本信息：

pLVX-MetLuc 载体名称:

质粒类型: 慢病毒载体；无启动子载体；荧光素酶报告载体 高拷贝/低拷贝: 高拷贝 克隆方法: 限制性内切酶，多克隆位点 启动子: 无

8088 bp 载体大小:

-- 5' 测序引物及序列:

-- 3' 测序引物及序列:

-- 载体标签:

载体抗性: 氨苄青霉素 筛选标记: 嘌呤霉素（Puromycin）

Stbl3 克隆菌株:

宿主细胞（系）: 常规细胞系（293、CV-1、CHO等）

慢病毒载体pLVX-MetLuc没有启动子元件； MetLuc是已知最小分子量的荧光素酶；

备注:

荧光素酶报告基因可以用来定性定量分析启动子及其它顺式转录元件的活 性。

稳定性: 稳表达

-- 组成型/诱导型:

病毒/非病毒: 慢病毒

pLVX-MetLuc载体质粒图谱和多克隆位点信息

pLVX-MetLuc载体简介：

pLVX-MetLuc is a promoterless, HIV-1-based, lentiviral vector that allows the functional analysis of different promoters and promoter/enhancer combinations inserted into its multiple cloning site (MCS). The vector encodes Metridia luciferase (MetLuc), a secreted reporter protein that can be easily detected in the medium surrounding the cells.

Metridia luciferase is a naturally secreted luciferase from the marine copepod Metridia longa. The human codon-optimized Metridia luciferase gene encodes a 219 amino acid (24 kDa) polypeptide that includes a 17 amino acid N-terminal signal peptide necessary for secretion (1). When a functional promoter is cloned into the MCS, located immediately upstream of the reporter, Metridia luciferase is expressed and secreted into the surrounding medium, where it is easily detected by the addition of a chemiluminescent substrate.

Lentiviral particles derived from pLVX-MetLuc allow you to monitor the activity of your promoter of interest in virtually any cell type, even primary cells. pLVX-MetLuc contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (2), leading to

increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (3). Finally, pLVX-MetLuc also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (4).In addition to lentiviral elements, pLVX-MetLuc contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase promoter (PPGK) for selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in

bacteria.

The pLVX-MetLuc vector, available as part of the Lenti-X Ready-To-Glow Secreted Luciferase Reporter System (Cat. No. 631746), can be used to monitor promoter activity in live cells as well as in vivo. A promoter must be cloned into the MCS, located upstream of the Metridia luciferase coding sequence. Without the addition of a functional promoter, the vector will not express MetLuc. Lentiviral particles derived from the vector allow you to monitor your promoter of interest in virtually any cell type, even primary cells. If required, stable transfectants can be selected using puromycin.

Propagation in E. coli

Recommended host strains: DH5α and other general purpose strains.

Selectable marker: plasmid confers resistance to ampicillin (100 μg/ml) in E. coli hosts. E. coli replication origin: pUC

References

1. Markova, S.V. et al. (2004) J. Bio. Chem. 279(5):3212-3217. 2. Zufferey, R. et al. (1999) J. Virol. 73(4):2886–2892.

3. Cochrane, A. W. et al. (1990) Proc. Natl. Acad. Sci. USA 87(3):1198–1202. 4. Zennou, V. et al. (2000) Cell 101(2):173–185.

pLVX-MetLuc载体序列

ORIGIN

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC

1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGTCGAGCTC AAGCTTCGAA TTCTGCAGTC GACGGTACCG 2221 CGGGCCCGGG ATCCCGCGAC TCTAGAATGG ACATCAAGGT GGTGTTCACC CTGGTGTTCA 2281 GCGCCCTGGT GCAGGCCAAG AGCACCGAGT TCGACCCCAA CATCGACATC GTGGGCCTGG 2341 AAGGCAAGTT CGGCATCACC AACCTGGAAA CCGACCTGTT CACCATCTGG GAGACCATGG 2401 AAGTGATGAT CAAGGCCGAC ATCGCCGACA CCGACCGGGC CAGCAACTTC GTGGCCACCG 2461 AGACCGACGC CAACCGGGGC AAGATGCCCG GCAAGAAGCT GCCCCTGGCC GTCATCATGG 2521 AAATGGAAGC CAACGCCTTC AAGGCCGGCT GCACCCGGGG CTGCCTGATC TGCCTGAGCA 2581 AGATCAAGTG CACCGCCAAG ATGAAGGTGT ACATCCCCGG CAGGTGCCAC GACTACGGCG 2641 GCGACAAGAA AACCGGCCAG GCCGGCATCG TGGGCGCCAT CGTGGACATC CCCGAGATCA 2701 GCGGCTTCAA AGAAATGGCC CCCATGGAAC AGTTCATCGC CCAGGTGGAC AGATGCGCCA 2761 GCTGCACCAC CGGCTGCCTG AAGGGCCTGG CCAACGTGAA GTGCAGCGAG CTGCTGAAGA 2821 AGTGGCTGCC CGACCGCTGC GCCAGCTTCG CCGACAAGAT CCAGAAAGAG GTGCACAACA 2881 TCAAGGGCAT GGCCGGCGAC AGGTAGTCTA GATAATTCTA CCGGGTAGGG GAGGCGCTTT 2941 TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT GGGCACTTGG CGCTACACAA 3001 GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAG GCGCCAACCG GCTCCGTTCT 3061 TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT CAGGAAGTTC CCCCCCGCCC 3121 CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC ACGTCTCACT AGTCTCGTGC 3181 AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT TTGGGGCAGC GGCCAATAGC 3241 AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA GGCTGGGAAG GGGTGGGTCC GGGGGCGGGC 3301 TCAGGGGCGG GCTCAGGGGC GGGGCGGGCG CCCGAAGGTC CTCCGGAGGC CCGGCATTCT 3361 GCACGCTTCA AAAGCGCACG TCTGCCGCGC TGTTCTCCTC TTCCTCATCT CCGGGCCTTT 3421 CGACCTGCAG CCCAAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG 3481 CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC 3541 GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT 3601 CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT 3661 GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC 3721 GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT 3781 GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA 3841 CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG

3901 CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG 3961 GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT 4021 GACCCGCAAG CCCGGTGCCT GACCGCGTCT GGAACAATCA ACCTCTGGAT TACAAAATTT 4081 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 4141 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 4201 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 4261 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 4321 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 4381 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 4441 TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 4501 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 4561 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA 4621 TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA TTAATTCTGC AGTCGAGACC TAGAAAAACA 4681 TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC 4741 ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC 4801 TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGAGGGGAC TGGAAGGGCT 4861 AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA 4921 CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG 4981 ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA 5041 GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT 5101 GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC 5161 GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT 5221 TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA 5281 AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC 5341 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC 5401 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT 5461 AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT 5521 AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGCCTTG ACATTGCTAG CGTTTTACCG 5581 TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 5641 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 5701 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 5761 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 5821 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 5881 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 5941 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 6001 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 6061 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 6121 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 6181 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 6241 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 6301 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 6361 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 6421 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG 6481 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC

6541 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 6601 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 6661 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 6721 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 6781 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 6841 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 6901 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 6961 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 7021 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 7081 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 7141 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 7201 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 7261 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 7321 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 7381 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 7441 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 7501 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 7561 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 7621 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 7681 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 7741 ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCAACT TGTTTATTGC 7801 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT 7861 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT 7921 CAACTGGATA ACTCAAGCTA ACCAAAATCA TCCCAAACTT CCCACCCCAT ACCCTATTAC 7981 CACTGCCAAT TACCTGTGGT TTCATTTACT CTAAACCTGT GATTCCTCTG AATTATTTTC 8041 ATTTTAAAGA AATTGTATTT GTTAAATATG TACTACAAAC TTAGTAGT //

pLVX-MetLuc其他相关慢病毒载体：

Tet-pLKO-neo pMD2.G

pLKO.1-TRC control

pCDH-MSCV-MCS-EF1-copGFP FUW-tetO-hOCT4 FUW

pLVX-AmCyan1-N1 pLVX-DsRed-Monomer-N1 pLVX-ZsGreen1-N1 pLVX-mCherry-C1 pLKO.1-puro pLVX-IRES-Puro

pLVX-EF1α-DsRed-Monomer-C1 pLVX-EF1α-mCherry-C1 pLVX-MetLuc Control

Tet-pLKO-puro pCMV-dR8.2-dvpr pLKO.1-hygro

pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro FUW-tetO-hSOX2 pLVX-AcGFP1-N1 pLVX-DsRed-Express2-C1 pLVX-PAmCherry-C1 pLVX-IRES-ZsGreen1 pLVX-mCherry-N1 pLentilox 3.7 pLVX-IRES-Neo pLVX-EF1α-AcGFP1-N1 pLVX-EF1α-IRES-mCherry pLVX-MetLuc

pPACKH1-GAG pLKO.1-GFP-shRNA pLKO.1-TRC FUW-tetO-hOKMS FUW-tetO-hKLF4 pLVX-AcGFP1-C1 pLVX-DsRed-Express2-N1 pLVX-PAmCherry-N1 pLVX-IRES-mCherry pLVX-tdTomato-C1 pLVX-Tet-On-Advanced pLVX-IRES-Hyg pLVX-EF1α-AcGFP1-C1 pLVX-EF1α-IRES-ZsGreen1 pLVX-Hom-Mem1

pLVX-Het-2 pSIH1-H1-CopGFP pCDF1-MCS2-EF1-copGFP pRSV-rev

pLVX-DD-AmCyan1 Control pLVX-DD-tdTomato Control pLVX-TetOne-Puro-Luc pLVX-TetOne-Luc pLVX-rHom-1 pLVX-PTuner pLVX-Het-1

pCDH-CMV-MCS-EF1-RFP-T2A-Puro pCDH-MCS-T2A-Puro-MSCV pCDH-EF1-MCS-T2A-copGFP pLVX-TRE3G-mCherry pLenti6.3/V5-GW/EmGFP pLenti6.3-MCS-IRES2-EGFP VSV-G

pcDNA6.3-EmGFP-NC- II pLenti6.3-BveI miR pGIPZ FUGW pMDLg/pRRE

pLVX-TRE3G-Luc Control pSico pGensil-1 pPACKH1-REV

pLKO.1-puro-GFP-siRNA pCDH-MSCV-MCS-EF1-Puro FUW-tetO-hMYC FUW-M2rtTA pLVX-AmCyan1-C1 pLVX-DsRed-Monomer-C1 pLVX-ZsGreen1-C1 pLVX-IRES-tdTomato pLVX-tdTomato-N1 pLVX-Tight-Puro

pLVX-EF1α-DsRed-Monomer-N1 pLVX-EF1α-mCherry-N1

pLVX-DD-AcGFP1-Actin pCDH-EF1-MCS-T2A-Puro pLOX-CWBmi1 pMDLg-pRRE

pLVX-DD-AmCyan1 Reporter pLVX-PTuner-Green pLVX-TetOne pLVX-rHom-Nuc1 pLVX-Hom-Nuc1 pLVX-PTuner2

pLVX-CherryPicker Control pCDH-CMV-MCS-EF1-Hygro pCDH1-MCS2-EF1-copGFP pWPXL

pLenti6.3-EmGFP-BveI miR pLenti6.3-MCS pLVX-shRNA2 pSico PGK Puro pcDNA6.2-EmGFP-NC- I pLenti6.3-MCS-IRES2-DsRed2 pLP2 pFUGW pLentG-KOSM pLVX-TRE3G-IRES pSicoR

pLVX-EF1α-IRES-Puro pLVX-Het-Mem1 pPRIME-TREX-GFP-FF3 pCDH-CMV-MCS-EF1-copGFP pLOX-TERT-iresTK pCDH-EF1-MCS-(PGK-Puro) pLVX-Hom-1 pLVX-mCherry-Actin pLVX-CherryPicker1

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro pLVX-PTuner2-C

pLVX-DD-ZsGreen1 Control pCDH-UbC-MCS-EF1-Hygro pCDH-CMV-MCS-EF1-RFP

pPRIME-TET-GFP-FF3 pCDH-CMV-MCS-EF1-Puro pLOX-CW-CRE pLL3.7

pLVX-DD-tdTomato Reporter pLVX-CherryPicker2 pLVX-TetOne-Puro pLVX-rHom-Sec1 pLVX-Het-Nuc1

pLVX-DD-ZsGreen1 Reporter pLVX-Tet3G

pCDH-CMV-MCS-EF1-Neo pCDF1-MCS2-EF1-Puro pLVX-TRE3G-ZsGreen1 pLenti6/V5-GW/lacZ pLenti6.3-DsRed2-BveI miR psPAX2

pcDNA6.2-DsRed2-MCS1 miR pcDNA6.2-EmGFP-BsaI miR pLEX-MCS pLP1

pLOX-Ttag-iresTK pCMV-dR8.91 pCgpv pLVTHM

pCDF1-MCS2-EF1-copGFP pLVX-shRNA1

pcDNA6.2-DsRed2-BsmBI miR pLVX-TRE3G pLP/VSVG

pcDNA6.2-EmGFP-MCS1 miR pcDNA6.2-BsaI miR pTRIPZ LeGO-iC2 pLKO.3G pLVX-Puro pSicoR PGK Puro pLVTH