专利名称：Combinatorial DNA library for producing
modified N-glycans in lower eukaryotes
发明人：Tillman U. Gerngross,Stefan Wildt,Byung-Kwon Choi,Juergen Hermann Nett,PiotrBobrowicz,Stephen R. Hamilton,Robert C.Davidson
申请号：US12156936申请日：20080605
公开号：US20090209024A1公开日：20090820
摘要：The present invention relates to eukaryotic host cells having modifiedoligosaccharides which may be modified further by heterologous expression of a set ofglycosyltransferases, sugar transporters and mannosidases to become host-strains forthe production of mammalian, e.g., human therapeutic glycoproteins. The inventionprovides nucleic acid molecules and combinatorial libraries which can be used to
successfully target and express mammalian enzymatic activities such as those involved inglycosylation to intracellular compartments in a eukaryotic host cell. The processprovides an engineered host cell which can be used to express and target any desirablegene(s) involved in glycosylation. Host cells with modified oligosaccharides are created orselected. N-glycans made in the engineered host cells have a ManGlcNAccore structurewhich may then be modified further by heterologous expression of one or moreenzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yieldhuman-like glycoproteins. For the production of therapeutic proteins, this method may
be adapted to engineer cell lines in which any desired glycosylation structure may beobtained.
申请人：Tillman U. Gerngross,Stefan Wildt,Byung-Kwon Choi,Juergen HermannNett,Piotr Bobrowicz,Stephen R. Hamilton,Robert C. Davidson
地址：Hanover NH US,Lebanon NH US,Norwich VT US,Grantham NH US,9B White RiverJunction VT US,Enfield NH US,Enfield NH US
国籍：US,US,US,US,US,US,US
更多信息请下载全文后查看
因篇幅问题不能全部显示，请点此查看更多更全内容